Composite

Part:BBa_K4773015:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-02)


Lac Promoter+RBS+cotA+RBS+QsrR+RBS+catA+Term.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3367
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2644
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2805


Design Notes

Different restriction sites were added between each part to facilitate the connection of each part or further manipulation.


Source

cotA: From the genomic sequence of Bacillus pumilus WH4. QsrR: From the genomic sequence of Staphylococcus aureus. catA: From the genomic sequence of Escherichia coli.

References